Enzyme Activity Levels in Mammalian Cell Cultures

Enzyme activity levels in mammalian cell cultures.

Improved methods for growing animal cells in culture have led to their wide use as a tool in a variety of disciplines in biology. Cultures from many different anatomical origins are available and purification of several of them has been effected by the method of single clone isolation (1). The exact nature of the cultured cells and their relationship to the organ of their origin is, however, un...

Enzvme Activitv Levels in Mammalian Cell Cultures*

Improved methods for growing animal cells in culture have led to their wide use as a tool in a variety of disciplines in biology. Cultures from many different anatomical origins are available and purification of several of them has been effected by the method of single clone isolation (1). The exact nature of the cultured cells and their relationship to the organ of their origin is, however, un...

Biosafety of Mammalian Cell Cultures

Metabolic flux rewiring in mammalian cell cultures.

Continuous cell lines (CCLs) engage in 'wasteful' glucose and glutamine metabolism that leads to accumulation of inhibitory byproducts, primarily lactate and ammonium. Advances in techniques for mapping intracellular carbon fluxes and profiling global changes in enzyme expression have led to a deeper understanding of the molecular drivers underlying these metabolic alterations. However, recent ...

Mammalian cell cultures for biologics manufacturing.

Biopharmaceuticals represent a growing sector of the pharmaceutical industry, and are used for a wide range of indications, including oncology and rheumatology. Cultured mammalian cells have become the predominant expression system for their production, partly due to their ability to complete the posttranslational modifications required for drug safety and efficacy. Over the past decade, the pr...